Journal of Photosciences

Cloning and characterization of the psbA Gene from Panax ginseng (Characterization of the psbA Gene from P. ginseng)

Volume 10(2003), 245-249 page

ÀúÀÚ: Won-Kyu Lee and Gun-Sik Tae

   10-3-245.pdf (144.3K)

Keywords: psbA  photosystem II  Panax ginseng  transcription  light intensity


The psbA gene of photosystem II was cloned and characterized from the P. ginseng chloroplast. The psbA gene is composed of 1,062 nucleotides. The overall amino acid sequence shows 99% and 98% identities to dicots and
monocots of higher plants, respectively. Southern blot analysis revealed that a single copy of the psbA gene existed in the chloroplast genome. Northern blot analysis of the in vivo accumulation of the psbA transcript, after being
grown under the different intensities (5%, 10%, 20%, and 100%) of daylight, indicated that the steady-state level of the psbA transcript was not significantly affected by light intensity.