Journal of Photosciences
Regiospecific Protein Perturbation on F NMR Shifts and Photoisomerizationof Fluororhodopsins. An Interpretation Based on Recent Crystal Structures of Rhodopsin
Volume 10(2003), 81-87 page
ÀúÀÚ: Leticia U. Colmenares and Robert S. H. Liu
10-1-81.pdf (271.4K)
Keywords:
Rhodopsin photoisomerization F NMR protein perturbation
Based on structural information provided by recently reported crystal structures of rhodopsin, we present rationales for the regiospecific protein perturbation on the previously reported 19F chemical shifts of the vinyl and trifluoromethylrhodopsins and their photoproducts. The crystal structures also suggest that H-bonding is a likely cause for the earlier reported regiospecific photoisomerization of the 10-fluororhodopsins. Photoisomerization was
revealed by chemical shift of the photoproducts. Additionally, possible use of 3-bond F,F coupling constants for following photoisomerization of retinal-binding proteins is discussed.
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